Artificial Hematopoietic Stem Cell Niche: Bioscaffolds to Microfluidics to Mathematical Simulations

Geeta Mehta

Associate Professor

mehtagee@umich.edu

3044 NCRC, Building 28

T: (734) 763-3957

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Maruti Didwania, Anjani Didwania, Geeta Mehta, Grzegorz W Basak, Satoshi Yasukawa, Shuichi Takayama, de, Necochea-Campion, Rosalia, An Srivastava, and Carrier, E (2011)

CURRENT TOPICS IN MEDICINAL CHEMISTRY, 11(13):1599-1605.

Due to the recent advancements in stem cell biology and engineering,scientists have been increasingly interested in creating in vitro nichesfor embryonic and adult stem cells, and, following induction anddifferentiation with the appropriate media, the production of largescale blood production. This artificially created niche forhematopoietic cells will be composed of three materials: the stem cellsthemselves, the scaffold surrounding the stem cell, and the media usedto expand and differentiate the stem cells. This paper will examine therecent advancements in technology for each of these relating to thedevelopment of an artificial stem cell niche. Many key aspects of theartificial niche need to be improved on before we can scale up theengineered device for large scale blood production including moreefficient methods of retrieval of the embroid bodies produced from themicrofluidic channels. The current state of experimental methods such asthese as well as relevant discoveries in related fields that could beapplied to artificial niche technology is described in this paper.Furthermore, we present a mathematical model to describe cell expansionin the artificial hematopoietic stem cell niche in order to design andoptimize a scaled-up bioreactor. The mathematical model describes thedynamics of expansion, and maintenance of homeostasis in the bioreactor.

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