Quantitative Inference of Cellular Parameters From Microfluidic Cell Culture Systems

Geeta Mehta

Associate Professor

mehtagee@umich.edu

3044 NCRC, Building 28

T: (734) 763-3957

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Khamir Mehta, Geeta Mehta, Shuichi Takayama, and Jennifer Linderman (2009)

BIOTECHNOLOGY AND BIOENGINEERING, 103(5):966-974.

Microfluidic cell culture systems offer a convenient way to measure cellbiophysical parameters in conditions close to the physiologicalenvironment. We demonstrate the application of a mathematical modeldescribing the spatial distribution of nutrient and growth factorconcentrations in inferring cellular oxygen uptake rates fromexperimental measurements. We use experimental measurements of oxygenconcentrations in a poly(dimethylsiloxane) (PDMS) microreactor culturinghuman hepatocellular liver carcinoma cells (HepG2) to infer quantitativeinformation on cellular oxygen uptake rates. We use a novel microchanneldesign to avoid the parameter correlation problem associated withsimultaneous cellular uptake and diffusion of oxygen through the PDMSsurface. We find that the cellular uptake of oxygen is dependent on thecell density and can be modeled using a logistic term in theMichaelis-Menten equation. Our results are significant not only for thedevelopment of novel assays to quantitatively infer cell response tostimuli, but also for the development, design, and optimization of novelin vitro systems for drug discovery and tissue engineering. Biotechnol.Bioeng. 2009;103: 966-974. (C) 2009 Wiley Periodicals, Inc.

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