Hard Top Soft Bottom Microfluidic Devices for Cell Culture and Chemical Analysis

Geeta Mehta

Associate Professor

mehtagee@umich.edu

3044 NCRC, Building 28

T: (734) 763-3957

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Geeta Mehta, Jay Lee, Wansik Cha, Yi-Chung Tung, Jennifer J Linderman, and Shuichi Takayama (2009)

ANALYTICAL CHEMISTRY, 81(10):3714-3722.

We report fabrication and characterization of microfluidic devices madeof thermoplastic and elastomeric polymers. These hard-soft hybridmaterial devices are motivated by the combined need for large scalemanufacturability, enhanced barrier properties to gas permeation andevaporation of aqueous solutions compared to poly(dimethyl siloxane)(PDMS) devices, and compatibility with deformation-based actuation.Channel features are created on rigid polymers such as polyethyleneterephthalate glycol (PETG), cyclic olefin copolymer (COC), andpolystyrene (PS) by hot embossing. These ``hard tops'' are bonded toelastomeric ``soft bottoms'' (polyurethane (PU) or PDMS-paryleneC-PDMS) to create devices that can be used for microfluidic cell culturewhere deformation-based fluid actuation schemes are used to perfuse andrecirculate media. The higher barrier properties of this device comparedto PDMS devices enable cell culture with less evaporation and creationof hypoxic conditions.

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