Microscope, Nikon SMZ-2T Stereomicroscope

Prepared by:

Ying Qi & Justin Scanlon


Dow 2224


The following materials and equipment associated with this procedure present exposure or physical health hazards. Safety precautions are prudent and mandatory.



Prior to performing this procedure, the following safety equipment must be accessible and ready for use: (e.g. chemical fume hood, biological safety cabinet, laminar flow hood, chemical spill kits)


Protective Equipment:

Prior to performing this procedure, the following personal protective equipment must be obtained and ready for use: (e.g. acid resistant gloves, safety eyewear, lab coat, chemical splash apron)


Waste Disposal:

This procedure will result in the follow regulated waste which must be disposed of in compliance with environmental regulations.



In the event that a hazardous material spills during this procedure, be prepared to execute the emergency procedure below.

Not applicable.







Detailed instructions:
  1. Remove the dust cover.
  2. Turn power on the Fiber Lite S80 illuminator to about 50% or as needed.
  3. Place the specimen onto the stage plate. *
  4. Turn both diopter rings on the observation tubes until the 0 line to the index. *
  5. Looking into the eyepieces of the observation tubes, adjust the interpupillary distance by moving the observation tubes, so that both the circular viewfields are brought into coincidence. *
  6. Set the zooming knob to 6.3x. Rotating the focus knobs, bring the specimen image into focus. If, beforehand, the distance between the specimen and the bottom of the microscope main body is made about 100mm, focusing will be facilitated. Rotate the zooming knob to 1 x. *
  7. Manipulating the right- and lefthand diopter rings one after the other, bring the images into focus independently.
  8. Repeat the above procedures from 4. to 7. to make sure of exact focusing, and, then the image will keep its sharpness all the
    time of continuous magnification changes by zooming. *
  9. Rotate the "optical-path change-over ring" from allowing you to view through both eyepieces to seeing only out of one. This allows you to view/capture images using the Cohu high performance CCD camera and the ImageJ software located on the Apple Computer sitting next to the microscope (see the associated SOP).
  10. When finished, rotate the "optical-path change-over ring" to its initial position.
  11. Clean up area and reposition the dust cover.

*Taken from the instructions provided by Nikon Corporation.
Nikon Stereo Manual1
Detailed diagram from Nikon International.


Last Revision:

May 22, 2024